FastQCFastQC Report
Mon 27 Feb 2023
SRR3127177.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127177.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139623
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121261.0640360891277203No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG34220.3002747399798003No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG28200.24745025328551637No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC26780.2349899923044726No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC13470.11819698268637961No Hit
AGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT11660.10231453735138726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT40700.086.143931
AGGGATG27050.081.658675
GGTAAGG7100.078.2143551
AGGGTAC4450.074.9842455
AGGGAAT18250.074.937955
GGATGGC20200.074.4509667
ACGGGTA950.074.206064
GTAGGGC6550.073.9039463
ATAGCGG2550.073.821461
GTAGGGT3100.072.7698063
AAGGGCG4600.072.5391164
TAGCGGG6750.071.7519762
ATGGCAC19000.071.732529
GATGACC15350.071.337728
GGGAATG21800.070.9269266
TAGGGTA3250.070.857274
GTACGGG2000.070.532892
GAGGGAT18650.070.306764
AGCAGGG20200.070.300112
AAGGGCA15450.070.267624
Produced by FastQC (version 0.11.5)