FastQCFastQC Report
Mon 27 Feb 2023
SRR3127166.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127166.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470111
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT455561.8442895886055324No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT40010.16197652656095213No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36790.1489406751356518No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT30010.12149251592337348No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT28710.11622959454048827No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG27970.11323377775330744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT136650.088.090471
ATAGGGC14500.073.448223
TAGGGCA11300.071.728354
GTAGGGC10700.071.3464053
ATAGGGA20350.069.701633
AGATAGG13000.069.637891
GAATAGG12450.068.548341
TAGAGGG44450.068.381342
GTACGGG5950.068.1131742
ATAGCGG5850.067.703511
TATAGGG20550.067.419442
AGGGAAT24200.067.384145
AGTAAGG16250.067.026471
AGTAGGG38400.066.391992
ATAGAGG19300.066.206361
AAGAGGG88150.066.183232
AGGGATG33500.065.932065
ACGGGAT4950.065.68784
AGGGCAT20350.065.583955
GAGGGAT27700.065.4970554
Produced by FastQC (version 0.11.5)