SRR3127159.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3127159.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1300801
Sequences flagged as poor quality	0
Sequence length	100
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11309	0.8693874005324411	No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	7271	0.5589632849298241	No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG	3823	0.29389583802595476	Illumina PCR Primer Index 8 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG	2878	0.2212482923982992	Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	2553	0.1962636867591584	Illumina PCR Primer Index 8 (95% over 23bp)
AAAGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT	1873	0.14398820419110994	No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC	1606	0.12346238971218504	No Hit
AGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT	1408	0.10824099919972385	Illumina PCR Primer Index 8 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCAGG	1341	0.10309032665257792	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTAGGG	335	87.018814	2
CGTTTTT	4130	85.12449	1
ATAGCGG	240	84.32207	1
CGCTACG	1285	83.02113	3
AGGGATG	4490	81.53743	5
GTACGGG	290	81.06591	2
TAAGGGA	2130	80.31334	3
TCCGCTA	1345	79.43055	1
GGATGGC	3260	79.14448	7
TACGACC	1360	78.788315	6
AAGGGAT	4345	78.52586	4
GCTACGA	1365	78.49972	4
GATGACC	2130	78.106926	8
TAGGGCA	785	77.82867	4
ACGACCA	1335	77.799515	7
CTACGAC	1380	77.64646	5
ACGGGTA	230	77.646454	4
ACGGGAT	755	77.18637	4
GGTAAGG	945	76.69603	1
CGACCAA	1365	76.08964	8