FastQCFastQC Report
Mon 27 Feb 2023
SRR3127157.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127157.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075640
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108451.0082369566025808No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC51060.4746941355843963No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG42390.3940909598006769Illumina PCR Primer Index 5 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG34530.32101818452270275Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC30000.27890372243501543Illumina PCR Primer Index 5 (95% over 23bp)
AAAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT19480.18110148376780336No Hit
AGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT13520.12569261091071363Illumina PCR Primer Index 5 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT12950.12039344018444834Illumina PCR Primer Index 5 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT11860.11025993826930944Illumina PCR Primer Index 5 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGCA11220.10430999219069577No Hit
AGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT10850.10087017961399725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA1150.090.14514
ATAGCGG1650.088.5639651
ACGGGAT4200.087.5114
CGGGTAT1000.084.589675
CGTTTTT36100.084.353791
CGCTACG9900.084.2471853
GCTACGA9850.084.196454
CGTAGGG2600.081.556112
GGATGGC18300.079.864567
GTAGGGT3200.079.51723
GGGCGAT3850.079.340966
TCCGCTA10700.079.299051
CTACGAC10500.079.2188955
ATAGGGA12350.078.980653
AGGGTAC5400.078.323775
TACGGGA3050.077.248043
GAGGGTA7450.077.165094
AGGGATG25950.076.603365
CGAAGGG9500.076.3861162
AAGGGTA8950.075.8152854
Produced by FastQC (version 0.11.5)