FastQCFastQC Report
Mon 27 Feb 2023
SRR3127156.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127156.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103199
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109610.9935650775608027No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54570.4946523700619743No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG39260.3558741441933867Illumina PCR Primer Index 5 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG32990.29903942987620546Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC29640.26867319495394754Illumina PCR Primer Index 5 (95% over 23bp)
AAAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT19100.17313286179556No Hit
GGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT14140.12817270501514233Illumina PCR Primer Index 5 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT13680.12400301305566812Illumina PCR Primer Index 5 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT12250.11104070979034608Illumina PCR Primer Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT1000.093.991253
ATAGCGG1700.091.3344351
CGTTTTT36600.086.3888551
ACGGGTA1900.084.0974354
GGGCGAT4150.083.799436
GGATGGC19150.082.7024847
TAGCGGG3300.082.6283342
ACGGGTC750.081.459084
GATGGCA18100.080.4897468
GCTACGA10550.079.736664
TACGACC10500.079.668776
CGCTACG10600.079.360543
CTACGAC10650.078.987955
TCCGCTA10850.078.9243851
GATAAGG6950.077.17731
CGTAGGG2200.076.9298252
AGGGATG27150.076.854725
AGGGTAG5150.076.653065
CGAAGGG9350.076.427022
AAGGGTA9550.076.275624
Produced by FastQC (version 0.11.5)