FastQCFastQC Report
Mon 27 Feb 2023
SRR3127154.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127154.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244530
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133021.068837231726033No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG43660.3508151671715427No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38820.31192498372879723No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG33420.2685351096397837No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC32950.2647585835616658No Hit
AGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT13760.11056382730830112No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGG6000.084.076371
CGTTTTT39250.083.600911
CGGGTAT1700.077.402045
GTAGGGT5250.077.19483
TAGGGCA5250.076.297194
ACGGGTA1550.076.007644
GGATAGG5700.075.267581
ATAGGGC7900.075.1609653
AGTAGGG17950.074.2969362
CGTAGGG3450.073.760452
GTATAGG3500.072.738971
GAATAGG6800.072.105071
TATAGGG8100.071.559782
TAGCGGG5250.070.91152
AAGGGTA8600.070.68714
GTACGTT200.002164170.491148
CATAGGG6450.070.139142
TAGAGGG17100.069.4469762
AGGGTAA6350.068.8265
AGGGCAT7800.068.683685
Produced by FastQC (version 0.11.5)