FastQCFastQC Report
Mon 27 Feb 2023
SRR3127153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295434
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136311.0522342319253624No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG43610.33664393554592514No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42690.3295420685268412No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC32060.24748462677372987No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG31730.24493721795166715No Hit
AGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT13900.10729994735355101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA1250.082.7137154
CGTTTTT39650.081.8982541
ATAGCGG2600.077.8330151
GGTAAGG7200.077.12911
TAGGGCG2200.076.9032444
ATAGGGC7800.075.31483
GGATAGG7150.075.035641
GTAGGGT5600.073.012313
AGTAGGG17200.072.708592
GAATAGG6950.072.4548951
TAGGGCA5750.071.924974
TATAGGG8800.071.590382
GTATAGG4250.070.869571
TAGGGAC7150.070.330324
CGAAGGG12600.070.1487352
TAATAGG3650.068.336341
ATAGAGG5650.068.302121
GTAGGGC5450.068.123273
AGGGACT30250.068.047725
TAAGGGA9400.067.9948353
Produced by FastQC (version 0.11.5)