FastQCFastQC Report
Mon 27 Feb 2023
SRR3127148.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127148.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182012
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129031.0916132831138772No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC75310.6371339715671246No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG46980.397457893828489No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG37990.32140113636748185No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC35500.3003353603855122No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT24290.2054970677116645No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCTC16700.14128452164614236No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC16650.1408615140963036No Hit
GGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT14930.1263100543818506No Hit
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT14470.12241838492333412No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT14270.1207263547239791No Hit
AAAGCGGCTGTGCAGACACTGTCTCTTATACACATCTGACGCCTCCAGGT13390.11328142184681712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAG750.087.7238545
CGTTTTT42650.086.742941
GGGCGAT4150.083.798186
ATAGCGG1900.081.854751
TACGGGT750.081.647833
TAGGGCG1500.081.647834
AGGGATG31400.081.567625
TTACGGG1850.081.481542
TCCGCTA14650.081.3891451
CGCTACG14400.081.124443
GCTACGA14400.081.124444
GGATGGC23750.080.138727
CGTAGGG1950.079.718712
ACGGGAT5500.079.649464
GATGACC16900.079.529888
AGGGAAT23050.078.290895
TACGGGA2950.078.24143
CGAAGGG9300.078.004332
ACGGGTA850.077.583914
AGGGTAT7300.077.251945
Produced by FastQC (version 0.11.5)