FastQCFastQC Report
Mon 27 Feb 2023
SRR3127141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142454
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200840.9374296951066394No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57270.26731028997588746No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG37190.17358599064437324No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG31880.14880132782314112No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC27900.1302244995691856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62650.082.085011
CGTAGGG4750.077.1934362
ATAGGGC11450.073.883093
TAGGGCA8750.073.048094
ACGGGAT4850.070.739044
ATAGGGA19800.070.7341463
AGGGATG27250.070.5398945
ATAGAGG14700.069.135511
AGTAGGG34150.068.276362
AGGGTAC8300.067.948715
GTAGGGA18050.067.697743
AGGGACT37200.067.3383265
GTACGGG5250.067.1554642
AATAGGG32000.066.987582
GAATAGG17150.066.940741
TATAGGG12400.065.9642
AGGGCAT19000.065.796995
GAGGGAT30750.065.720574
TAGGGAC10800.065.709964
AAGAGGG74850.065.567422
Produced by FastQC (version 0.11.5)