FastQCFastQC Report
Mon 27 Feb 2023
SRR3127131.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127131.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3405585
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351561.032304288396854No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT75130.22060820681322008No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC69340.20360672248673867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT112900.085.416791
TAGGGCA20400.075.336274
AGGGAAT41950.073.60395
AGGGATG76500.073.474585
ATAGGGC23900.073.34743
TAAGGGA32250.071.98983
CGTAGGG10650.070.605382
ATAGGGA32850.070.531863
AGGGCAT33250.070.389055
TAGGGTA10500.069.826754
AGTAGGG52550.069.399512
ATAGCGG7850.068.8810351
GAGGGAT49850.068.164724
GTAGGGA27850.067.838253
CGAAGGG35000.067.809532
ATAGAGG24950.067.465841
TATAGGG27300.067.3102952
AAGAGGG119800.067.199792
AGAGGGA85200.067.186413
GTACGGG7650.066.962622
Produced by FastQC (version 0.11.5)