FastQCFastQC Report
Mon 27 Feb 2023
SRR3127126.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127126.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2903522
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246960.850553224669901No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT69120.23805571302714426No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG56650.1951078724390585No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG45570.15694732121885077No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC45100.1553285974757553No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29060.1000853446262849No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78950.082.903861
GTAGGGC14600.076.2906653
CGTAGGG8000.075.222112
ATAGGGC18150.074.574743
TAGGGCA15650.073.5745544
TAGGGCG5400.073.1074144
ATAGCGG5350.073.000191
AGTAGGG50750.072.6282352
GTAGGGA25600.071.7815253
AGGGATG46650.069.614925
AGGGACT47700.069.3633655
TAGCGGG11950.068.848692
GGAGCTA47550.068.494969
GAATAGG17950.068.156711
GTAGGGT13250.068.102223
ACGGGAT8600.067.7640154
ATAGGGA26350.067.241383
CGAAGGG27950.066.946332
TAGAGGG50150.066.4661942
GGTAAGG19100.066.2702561
Produced by FastQC (version 0.11.5)