FastQCFastQC Report
Mon 27 Feb 2023
SRR3127124.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127124.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2350995
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201930.8589129283558663No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG36640.155848906526811No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG33020.14045117067454418No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32250.13717596166729407No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC30350.1290942771039496Illumina Single End Adapter 2 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29780.12666977173494626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67250.082.4537351
TAGGGCA12750.078.3557364
AGGGATG39250.074.711535
TAGACGG3000.072.298931
ATAGGGC15700.072.0371863
TAGAGGG39000.071.8948062
GTAGGGC12650.071.5246663
AGGGCAT21400.071.149845
AAGGGAT41450.070.373844
AGGGAAT29850.069.900675
TATAGGG21800.069.821822
ATAGGGA22250.069.6804663
AGTAAGG16300.069.1362151
AGTAGGG39800.069.028852
CGTAGGG5950.068.904472
TAAGGGA23700.068.59873
ATAGCGG4750.068.493721
GAGGGAT33500.067.240084
GGTAAGG14650.066.945441
CGAAGGG21300.066.814772
Produced by FastQC (version 0.11.5)