FastQCFastQC Report
Mon 27 Feb 2023
SRR3127121.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127121.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2876713
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT665162.3122223176243164No Hit
CGTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT70340.24451518104169584No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT60420.2100313795641067No Hit
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT51870.18030995792767648No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47530.1652232947812312No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG40560.14099425281562672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT217650.087.742211
ATAGGGC17750.076.981283
ATAGCGG6850.071.572761
AGTAGGG42600.070.235572
AGAGGGC43300.069.860643
AGGGATG44900.069.810965
TAGAGGG48600.068.06032
TAGGGCA14100.067.8363344
ATAGAGG18900.067.3452451
GTAGGGA20400.066.9812553
GTACGGG6000.066.751452
TAGCGGG15800.066.502932
AGGGACT37800.066.139975
AGGGAAT30000.066.1051565
AGAGGGA60400.065.996193
GTAGGGT10300.065.87353
TGTAGGG18300.065.6571662
GAATAGG15950.065.614111
AGTAAGG19600.065.42111
AGGGCAT26100.065.179685
Produced by FastQC (version 0.11.5)