FastQCFastQC Report
Mon 27 Feb 2023
SRR3127118.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127118.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492632
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51811.0516978190616932No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG17100.34711508793582224No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG14700.29839718085711037No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC13070.26530960229948525Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT6150.12483963688919925No Hit
CGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT5410.1098182822065964No Hit
GGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT5230.10616443917569302No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC5130.10413452638074669No Hit
AGAGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTT4970.10088666590883255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT18500.085.695071
TACGGGG1800.083.830153
GTACGGG1250.082.991852
CGTAGGG800.082.520312
CGCTACG1100.081.4486163
CGGGAAT1600.079.298545
TACGACC1150.077.638516
ATAACGG551.8189894E-1277.209031
CGAAGGG3300.077.161852
CGGGTAT551.8189894E-1276.895555
TTACGGG800.076.6262
AAGGGAC4750.076.4398654
AGGGATG6250.075.938635
AAGCGGG4050.075.682
AGTGCGG1000.075.493271
ACGGGTA506.91216E-1175.447144
GACGAGG1450.074.842471
TCCGCTA1200.074.706891
GCTACGA1200.074.661234
CTACGAC1200.074.403575
Produced by FastQC (version 0.11.5)