FastQCFastQC Report
Mon 27 Feb 2023
SRR3127099.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127099.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826494
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322351.1404588157625666No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC79410.280948765502421No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC31110.11006568561617325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115150.087.659921
AGGGATG102700.080.541325
ATAGCGG5750.080.203991
ACGGGAT16150.080.027024
TAGGGCA24650.078.9332354
AAGGGAT89650.077.1150054
CGGGATG17450.075.9504245
GTAGGGC17350.075.846413
GGATGGC65650.074.7381447
TAGCGGG14300.074.3181462
AGGGAAT50600.074.211775
GCGGGAT18650.074.087514
TAGGGAT39200.073.9734044
AAGGGCG15650.073.574664
GAGGGAT72950.073.379544
ATAGGGA43950.073.357033
AGAGGGA100400.073.2583543
ATAACGG4050.073.202051
AGGGATC35250.072.7964255
CGAAGGG25700.072.4576952
Produced by FastQC (version 0.11.5)