FastQCFastQC Report
Mon 27 Feb 2023
SRR3127061.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127061.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332820
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35741.0738537347515174No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG15600.4687218316206959TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG12580.3779820924223304TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC10820.32510065500871344TruSeq Adapter, Index 22 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT4930.148128117300643No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4860.1460248783126014No Hit
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT4760.1430202511868277No Hit
GGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT4530.13610960879754821TruSeq Adapter, Index 23 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT4010.12048554774352503No Hit
AGAGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT3930.11808184604290607No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT3870.11627906976744186No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATG3640.10936842737816237No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG3540.10636380025238867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGG301.285116E-894.3944551
TTAGCGG409.094947E-1294.394451
TTACGGG254.8053516E-794.351732
ATCCGTT156.902777E-493.983179
GGCGATC156.902777E-493.983177
GTATAGG1100.090.10381
AGGGCGA1150.085.810725
CGTAGGG550.085.774312
CGTTTTT13150.084.7037661
GGCGATA401.035005E-982.2352757
TAGGGCA1400.080.860724
GCCGATG353.860987E-880.557018
TAAGCGG650.079.872221
TAGCGGG1300.079.8360752
GGGAATG7750.078.824596
GGATACG301.4554098E-678.319318
ATAGGGC2000.077.8284453
AGGACGG850.077.73661
GGGCGAT1400.077.200466
TCGTCTC551.8189894E-1276.8953255
Produced by FastQC (version 0.11.5)