FastQCFastQC Report
Mon 27 Feb 2023
SRR3127056.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127056.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481449
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64721.3442753022646219No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG21680.45030730150026277TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC18480.38384127913860033No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG18160.37719467690243413TruSeq Adapter, Index 22 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT7020.14580983655589688No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT6820.14165571015829298No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6490.13480140160224655No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCC6010.12483149824799719No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT5940.12337755400883582No Hit
GATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC5800.1204696655305131No Hit
ACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG5580.11590012649314879No Hit
GGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT5520.11465388857386763No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATG5290.10987664321662316No Hit
ACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT5090.10572251681901924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG353.583409E-1094.0289841
ACTCGCG353.583409E-1093.9996959
ATTCCGA156.899848E-493.999689
GGTAAGG2550.088.497871
CGTTTTT21150.085.359641
CGTAGGG1100.081.181562
TTGACGG353.851528E-880.5962751
ACTACGG301.4521866E-678.357491
TATAGGG4550.077.472272
AGGGAAT10500.077.4378365
TAGGGCA2250.077.2886354
ATAGGGC3300.076.908843
GTAGGGC1600.076.374753
AGTAGGG6550.076.060822
TACGGGG1800.075.721973
TAACCGG750.075.223191
TACGGGT255.5561875E-575.199753
CGGGACT1000.075.199755
TAGGGCG750.075.199754
AATCGGG255.5561875E-575.199752
Produced by FastQC (version 0.11.5)