FastQCFastQC Report
Mon 27 Feb 2023
SRR3127043.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127043.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331298
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228950.982070932158823No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT59470.25509394337403457No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50890.21829041160761087No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG45360.1945697203875266No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG36820.15793776685777622No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC33330.14296756570803046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67700.085.245651
TAGGGCG4150.084.032684
AGTACGG2600.077.978451
AGGGATG51400.076.068155
AGGGTAC10850.073.198075
TAAGGGA23400.072.9053
AAGGGCG12100.072.052814
GGTAAGG13300.071.965421
AGTAGGG36500.071.017012
TAGCGGG11950.070.9900052
AAGGGAT46650.070.714914
AGGGAAT33850.070.525745
GGGCGAT9400.070.491046
TAGGGCA14850.069.816944
ATAGGGA22450.069.692543
AGATAGG12950.069.541341
TATAGGG18050.069.453972
AGGGCAT21550.069.3461765
AACGAGG11100.069.237931
ATAGGGC17250.069.1187743
Produced by FastQC (version 0.11.5)