FastQCFastQC Report
Mon 27 Feb 2023
SRR3127034.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127034.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743560
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393851.43554360028576No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40070.1460511160681742No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG30510.111205878493636No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG29050.10588432547493037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118300.085.357661
ATAGGGC17000.074.283993
TAAGGGA23000.072.319633
TAGGGCA13550.071.289224
CGTAGGG8200.071.257912
AGTAGGG46500.070.936592
AGTAAGG18400.070.712971
ATAGGGA23700.070.5812453
AGGGATG41450.069.83945
GTAGGGC14450.068.8056263
GTAGGGA20850.068.7031863
GAATAGG19950.068.054581
GTACGGG6150.067.4268342
AGGGAAT29950.067.3138965
AGATAGG14750.067.117391
GATAGGG33450.066.914882
GAGGGAT28850.066.148284
ATAAGGG36450.065.415132
AATAGGG42150.065.177282
AAGGGGC79150.065.12924
Produced by FastQC (version 0.11.5)