FastQCFastQC Report
Mon 27 Feb 2023
SRR3127017.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127017.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170194
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222381.02470101751272No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54500.25112962251301035No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG48110.22168525025873265No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG46290.21329890323169265No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC40530.18675749725600568TruSeq Adapter, Index 16 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31470.14501007744008138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69750.083.989671
AGGGATG44550.078.494125
GGTAAGG11750.078.427841
TAGGGCA12700.076.2386864
AGTAGGG33750.075.754252
CGTAGGG5700.074.20782
ACGGGAT5700.073.3883364
AGGGTAA8800.072.638725
ACGGGTA3900.072.3100364
GGATGGC32400.071.51617
AGGGAAT31800.071.24135
CGGTATA200.00216296270.502285
ATAGGGC13300.070.322343
TACGAGG3750.070.2115861
GAGGGAT31800.070.058874
AAGGGTA16300.069.78144
ATAGGGA22250.069.706913
TAAGAGG13700.069.3238751
AAGGGAT48500.069.29094
GAATAGG14600.069.23691
Produced by FastQC (version 0.11.5)