FastQCFastQC Report
Mon 27 Feb 2023
SRR3127015.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127015.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818540
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171700.9441639996920607No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG43840.24107250871578298No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43270.23793812618914073No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG38500.21170829346618714No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC36090.1984559041868752TruSeq Adapter, Index 16 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25360.13945252785201318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCA10050.083.7059564
CGTTTTT55550.083.1165851
TAGACGG2800.077.321911
ACGGGTA3350.077.159134
AGGGATG33400.075.27955
TAAGGGA17100.074.48053
AGTAGGG27800.074.259482
AGGGAAT26750.073.438185
ACGGGAT5500.072.63164
GAATAGG10700.072.5776441
CGTAGGG3850.072.064852
AAGGGTA12150.071.945914
AGGGCAT16800.071.89415
GGTAAGG9900.071.786781
TACGCGG1650.071.311371
TAGCGGG8050.071.268262
ATAGAGG10850.071.140491
AGATAGG11400.071.011121
GTAGGGA14500.070.981553
TAGGGTA6150.070.304344
Produced by FastQC (version 0.11.5)