FastQCFastQC Report
Mon 27 Feb 2023
SRR3127000.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127000.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768157
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282071.0189812210795848No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33940.12260865261616302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96300.084.83881
CGTAGGG6050.075.390812
ACGGGTA4650.074.790324
TAGGGCA13850.072.27644
AGTAGGG43900.071.015162
ATAGGGC17100.070.907183
GGTAAGG17050.069.558031
TAGGGCG5400.068.754324
AGATAGG17350.067.812791
AGGGATG44800.067.557645
ATAGGGA27650.067.4779743
AAGGGTA23900.067.0537644
AGGGTAC14350.066.8105245
AGGGTAG16350.066.686335
AGGGCAT22950.066.552955
TAGAGGG52850.066.551622
GTAGGGC14000.066.466653
GCATAGG7300.066.402661
ATAGAGG20000.066.357531
TAGGGTA8450.066.1845864
Produced by FastQC (version 0.11.5)