FastQCFastQC Report
Mon 27 Feb 2023
SRR3126982.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126982.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2613436
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT663322.5381145740703044No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT63720.2438169520891271No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT53040.2029512105901962No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT49380.18894665872820304No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG36540.13981593580252205No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30910.1182734147689096No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC28150.10771260516806228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT199100.089.74391
ATAGGGC15100.074.694013
AGTAGGG39850.073.509572
AGGGATG33600.072.730325
TAGGGAT14650.068.9687354
TAGCGGG14000.068.51512
GTAGGGA17650.068.428953
AGAGGGC41550.068.428313
AGGGAAT27200.068.246385
ATAGCGG6150.068.118471
AGGGCAT19900.068.012835
GTAGGGC11750.067.992733
AGATAGG12150.067.7971951
GAATAGG14650.067.473251
ATAGGGA20500.067.3976753
GTAGGGT7950.067.389033
ATGCGGG12500.066.580562
TAGGGCA13050.065.9014
GAGGGAT28500.065.792974
ATAAGGG36700.065.597622
Produced by FastQC (version 0.11.5)