FastQCFastQC Report
Mon 27 Feb 2023
SRR3126971.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126971.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123672
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209590.9869226509555148No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG56490.26600152942638977No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG41730.19649927107387583No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC41390.19489827054272033No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23160.10905638912223733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62700.085.705311
CGTAGGG4550.079.794752
GGTAAGG12750.075.856921
AGGGATG29300.075.701545
TAGGGCA9150.075.232624
ATAGGGC12100.074.815283
AGTAGGG29900.073.486862
TAGCGGG9050.071.899442
AGGGCAT19250.071.282425
ATAGCGG3850.071.075321
ATGCGGG9100.070.468092
TAGAGGG32050.070.175472
TAGGGTC5350.069.62224
AATGCGG4550.069.4728241
TAAGGGA19550.069.4576643
ATAGGGA21250.069.448023
TATAGGG18950.068.674392
AGGGAAT27300.068.337355
TAGGGAT15750.068.25414
TAACGGG7400.068.178442
Produced by FastQC (version 0.11.5)