FastQCFastQC Report
Mon 27 Feb 2023
SRR3126944.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126944.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238098
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207160.9256073683994177No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG29970.13390834538970145TruSeq Adapter, Index 22 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26220.11715304691751657No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG24300.10857433409975792TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC24030.10736795260976062Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71250.081.182381
TAGGGCA10400.075.6659244
ATAGGGC10600.074.238263
TAGACGG3150.073.3311
ACGGGTA2250.073.299794
AGTAGGG32900.071.7561342
GTAGGGC10750.070.134023
AGGGAAT25400.069.3807755
CGTAGGG5450.069.168872
AGGGCAT21100.068.820475
GTAGGGT8500.068.741653
ACGGGAT6200.068.401884
AGGGTAC8050.068.301715
TAGGGTA8450.066.917814
ATGAGGG33650.066.5159452
GAATAGG14250.066.163311
AAGAGGG78200.066.162632
TAGCGGG11400.066.135152
AAGGGTA12550.066.082454
ATAGAGG15100.065.873021
Produced by FastQC (version 0.11.5)