FastQCFastQC Report
Mon 27 Feb 2023
SRR3126881.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126881.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284511
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185570.8122963732720043No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40740.17833138032603038No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35320.1546063906017524No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26850.1175306225270966No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT25510.1116650346616847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70400.084.427121
ACGGGTA2600.077.724274
AGGGATG28250.073.197425
TAACGGG7550.072.205892
CGAAGGG23850.072.119762
GGTAAGG12550.070.871131
ATGTAGG7150.069.7671661
ATAGGGC13550.069.713723
AGTAGGG35950.069.546342
TAAGGGA19550.069.472463
ATAGGGA20250.068.9275743
TAGGGCG4850.068.798374
GTACGGG5250.068.032412
GAATAGG14350.067.556411
AAGGGCA33400.067.539264
ATAGAGG15200.067.493851
TGTAGGG18400.067.4291462
AGGGTAC8950.067.212265
AGAGGGC31100.067.094073
ACGGGAT6250.066.922414
Produced by FastQC (version 0.11.5)