FastQCFastQC Report
Mon 27 Feb 2023
SRR3126851.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126851.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265095
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68520.5416194040763737No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA50830.40178800801520836No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39590.31294092538505014No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG35530.2808484738300286No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG30190.23863820503598546No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC26660.21073516218149624No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23010.18188357396084878No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT21820.17247716574644592No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA15540.1228366249174963No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT14120.11161217141795676No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGT13720.10845035353076252No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTACG201.8298593E-594.0837551
ATAGCGG2250.087.811511
CGTTTTT33450.086.208281
CGGGTAA600.086.158225
CTACGAC6400.085.913455
ACGGGTA1500.084.5917054
CGTAGGG2800.083.920362
TAGCGGG6400.083.710552
TCCGCTA6750.083.6300051
CGCTACG6550.083.2284853
AGGGATG22600.082.5538565
CGAAGGG11550.081.7841952
TAGGGCG2250.081.458694
ACGGGAT5400.080.936514
TCGATGG353.84116E-880.643221
AGGGCGC2800.080.563545
GCATAGG3050.080.2025451
GCTACGA6850.079.583444
AGTAAGG8200.078.594361
AGCGGGA8650.078.235113
Produced by FastQC (version 0.11.5)