FastQCFastQC Report
Mon 27 Feb 2023
SRR3126767.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126767.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118757
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197620.9327166824699575No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44140.2083296951939274No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38350.18100235185063696No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26500.12507333309105292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76850.086.643481
AGGGATG44900.075.9905855
ACGGGAT7500.074.568474
ATAGCGG4700.074.089591
CGTAGGG6400.073.432712
TAGGGCG4750.073.216274
CGAGGGA13950.072.095653
CTACGAC9700.071.706665
TACGACC9750.071.338936
TAACGGG7000.071.166782
GAGGGAT38750.071.071494
GTAGGGC9000.071.0175863
AAGGGAT50650.070.982544
CGAAGGG21150.070.2176362
TAGGGCA10950.069.95894
AGGGATT33150.069.751115
AGAGGGA57300.069.634193
TAGCGGG11150.069.547032
GGTAAGG12600.069.464951
TAAGGGA21550.069.132383
Produced by FastQC (version 0.11.5)