FastQCFastQC Report
Mon 27 Feb 2023
SRR3126699.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126699.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2150769
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272391.2664772460454843No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT50700.23572963902678531No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26720.12423463421687778No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT24000.11158799480557884No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT22270.10354436018001002Illumina PCR Primer Index 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92650.086.759271
ATAGCGG5150.075.848021
TAGCGGG11500.074.834742
GGTAAGG12800.074.270251
ATAGGGC11850.073.372063
CGTAGGG6450.072.9105452
AGTAGGG34550.071.32362
AGGGCAT18500.070.877735
AGGGTAC9750.070.858195
GAATAGG14000.070.59351
AGGGATG28450.069.381615
AATAGGG31600.068.606282
AGTAAGG13900.067.715581
TAGGGCA11800.067.310394
GAGGGAT24450.066.50814
TAGAGGG37200.066.495592
AGAGGGC30350.066.4317863
ATAGAGG15500.065.583631
AGAAGGG78600.065.455292
ATGAGGG31900.065.4549262
Produced by FastQC (version 0.11.5)