FastQCFastQC Report
Mon 27 Feb 2023
SRR3126697.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126697.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292769
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271521.184244902124898No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53260.2322955343516944No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35550.15505268956445242No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25760.11235322878144288No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC25000.10903845960931957TruSeq Adapter, Index 15 (95% over 21bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT24590.10725022887172672No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG23140.10092599821438619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93950.086.7962651
CGTAGGG5950.078.423372
TAGCGGG12250.075.413182
TAGGGCG4750.075.413184
AGTAAGG14100.073.922391
GTAGGGC10700.073.122593
TAGGGCA11600.072.325144
TAAGGGA19500.071.545843
ATAGCGG4950.071.459321
ATAGGGC14900.071.1743553
AGTAGGG36800.071.084092
GGTAAGG13950.070.660211
ACGGGAT5650.070.0741964
ATAGAGG14850.069.871341
AAGGGAC21400.069.818864
TAGAGGG40600.069.4228442
AATGCGG4350.069.3894651
TAGGGAC10950.069.300924
AAGGGTA13950.068.9260254
AGAAGGG76750.068.719342
Produced by FastQC (version 0.11.5)