FastQCFastQC Report
Mon 27 Feb 2023
SRR3126696.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126696.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305420
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253611.100059858941104No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT52330.22698683970816597No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34200.14834607143166972No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC24900.10800635025288235TruSeq Adapter, Index 15 (95% over 21bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT24680.10705207727876048No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24660.10696532519020396No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG23680.10271447285093388No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94250.087.500711
CGTAGGG5800.074.577352
TAAGGGA17850.073.718843
ATAGGGC14750.073.6001363
AGGGTAC8950.073.512925
AGTAAGG14200.072.886211
GGGCGAT10650.072.369066
TAGCGGG11850.071.416952
AGTAGGG35800.070.786892
TAGGGTC5550.070.281954
GTAGGGC11850.070.1962053
AGGGATG25850.070.175495
AGGGCAT20100.069.67535
ATAGCGG5600.068.886931
AAGGGTA14400.068.861844
CGAGGGA12900.068.854253
GGTAAGG13600.068.49161
CGAAGGG24050.068.4226762
TAGGGCA11350.068.319834
ACGGGAT5400.067.882774
Produced by FastQC (version 0.11.5)