FastQCFastQC Report
Mon 27 Feb 2023
SRR3126694.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126694.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2098601
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258931.2338219604393594No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44080.2100446916779321No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27340.13027726566412576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95000.087.449761
ACGGGTA2000.077.788634
TAGGGCG3950.075.192684
AGTACGG3350.073.2287141
ATAGCGG3550.071.760971
ATAGGGC13700.070.888993
GGTAAGG12350.069.904811
CGTAGGG7500.069.774032
ACGGGAT4400.069.645464
AGGGTAC8250.069.492655
AAGGGAC20650.068.4909744
TAAGGGA16000.068.065053
GAGGGTA11400.067.822094
GAATAGG14900.067.756411
TGTAGGG15050.067.349462
GTACGGG5600.067.349462
AGGGCAT16950.067.093235
AGGGAAT25500.067.0801855
GTAGGGC9500.066.994993
TGAAGGG36600.066.594992
Produced by FastQC (version 0.11.5)