FastQCFastQC Report
Mon 27 Feb 2023
SRR3126693.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126693.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108022
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245411.1641719109193358No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42550.20184798830372735No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27230.12917322494736772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88400.087.047911
ACGGGAT4650.073.780964
ATAGGGC13350.073.5765463
ATAGCGG4100.073.4664461
TAGGGCG3400.073.260754
AGGGCAT17100.070.9084555
TAGGGCA11550.070.801374
GTAGGGC9550.068.8968053
AGAGGGC30250.068.515313
AGTAGGG33500.068.08472
TAGCGGG11200.068.022032
CGTAGGG6300.067.928732
CGAAGGG20700.067.247172
TAAGGGA17550.066.412373
ATGAGGG31350.066.3036352
AAGGGTA12000.066.188094
AGGGTAC7700.065.9185265
GAGGGTA11400.065.549094
TACGCGG2300.065.4809651
AGACGGG15300.065.4697652
Produced by FastQC (version 0.11.5)