FastQCFastQC Report
Mon 27 Feb 2023
SRR3126683.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126683.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933696
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319841.090228844433779No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57220.1950440672789546No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41530.14156204323829052No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG32160.10962281027073018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT122150.086.263011
AGGGATG38050.073.489685
GGTAAGG18050.072.7429661
TAGGGCA14000.071.837264
ATAGGGC20450.071.701013
AGTAGGG47200.070.992352
ACGGGAT8050.070.056654
GAATAGG20400.069.20781
ATAGGGA26650.068.2460863
CGAAGGG27800.067.789682
TAAGAGG21450.067.57521
ATAGCGG6200.067.555751
AAGGGTA18300.067.541184
AGGGCAT24950.066.680175
TAGGGCG6650.066.4309164
TAAGGGA24550.066.235163
ATAGAGG18900.066.2344361
AATAGGG46850.066.2061542
GTAGGGC12950.066.0489043
AGTAAGG18100.066.041831
Produced by FastQC (version 0.11.5)