FastQCFastQC Report
Mon 27 Feb 2023
SRR3126678.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126678.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384521
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55520.4010051129596445No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA53350.38533182234144514No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37210.26875720917198076No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT32120.23199359200763298No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG27700.2000691936055863No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG25730.18584044590150675Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG24980.18042340997355763RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC24720.17854550418520196RNA PCR Primer, Index 41 (96% over 26bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGACATC19060.13766493971561283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGGG10850.089.267472
AATGCGG2900.086.0272451
CGGGATA2050.084.823345
GACCGAT900.083.5497068
TCCGCTA7550.083.544141
CGTTTTT34150.083.529531
ACGGGTC1550.081.865244
CGCTACG7750.081.865243
ACGGGTA1150.081.733414
GCGAAGG5450.081.187561
CGTAGGG4250.080.7587662
GCTACGA7750.080.652434
CGGGTAA1050.080.5657965
ATAGCGG3450.080.499111
TAGCGGG8150.079.611762
GGGCGAT7450.079.4843756
AGGGCGA7400.079.386345
CTACGAC8000.078.1320345
ACGGGAT4100.077.945774
AGGGATG23400.077.926175
Produced by FastQC (version 0.11.5)