FastQCFastQC Report
Mon 27 Feb 2023
SRR3126659.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126659.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918001
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325711.6981742970936928No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37290.19442117079188176No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT28970.1510426741174796No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT25500.132950921297747No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA24280.1265901321219332No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT22530.11746604928777409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109750.088.638941
AGGGATG26250.075.733475
GTAGGGT4950.073.105663
AAGGGTA11450.073.060054
ATAGCGG5650.072.4701841
AGGGCAT15300.070.957345
GAGGGAT19850.069.843664
TAGCGGG12800.069.393262
GGTAAGG10000.069.1840441
ACGGGAT5300.069.164654
AGTAGGG32000.069.02612
AGGGTAG6100.068.570445
CGTAGGG5050.067.9355242
TAGGGCA9500.067.773894
ATAGGGC11250.067.674963
AGGGTAC8000.067.559225
AGAGGGC26450.067.340913
AGGGTAT9100.066.623145
AGAAGGG62750.066.581482
TGTAGGG13350.066.534362
Produced by FastQC (version 0.11.5)