FastQCFastQC Report
Mon 27 Feb 2023
SRR3126655.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126655.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895845
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367561.938766091109769No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38410.20260095102711456No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT29900.15771331517080775No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT26340.13893540874913296No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT25330.13360796900590502No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA24950.13160358573617567No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG19680.10380595460071894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT126250.089.232631
TAGGGCA8850.075.6386954
ATAGGGC11500.074.6057743
TAGGGTA5850.072.524644
AGTAGGG31550.072.170252
AGGGTAC8350.071.474485
AGGGATG24950.070.8192145
GTACGGG4000.070.7133942
AGGGAAT21500.070.5988855
AGTAAGG11850.070.067251
ACGGGAT4250.068.7704244
TAACGGG6050.068.5705642
CGTAGGG4950.068.5705642
AAGGGTA12750.068.400694
GTAGGGT7150.067.909443
TAAGGGA15800.067.727923
AGGGTAT10050.067.333345
AGGGACT25000.066.918125
GTAGGGA14400.066.783113
AGGGCAT12950.066.770075
Produced by FastQC (version 0.11.5)