FastQCFastQC Report
Mon 27 Feb 2023
SRR3126583.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126583.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373516
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113070.8232157470317055No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCA33450.24353556857000572No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG29790.21688862743499165No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29030.21135538282772096No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG28920.21055451847666862No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC28790.20960804242542494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTA4650.083.882444
CGTTTTT42650.083.183291
GGTAAGG7550.074.162411
ATAGGGA9850.072.519243
AGGGATG17750.072.0139165
GTACGGG2750.071.7680052
CGGATAT200.002164096670.491575
GTAGGGT5750.070.287243
AGTAGGG22700.069.140882
AGTAAGG9150.068.907661
ATAGAGG9650.068.750461
ACGGGAT3150.068.62674
ATAGCGG2950.068.585171
GTAGGGC6000.068.1418463
AGGGAAT16700.067.2554245
TAGGGAC5600.067.134834
AGGGACT21250.067.008455
AGAAGGG48700.066.868032
TAGGGCG2750.066.646574
ATGAGGG20450.066.6375352
Produced by FastQC (version 0.11.5)