FastQCFastQC Report
Mon 27 Feb 2023
SRR3126582.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126582.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527579
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145020.9493453366405273No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG33610.22002135405108345No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC33020.21615903334622957No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32540.21301680633211115No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG32520.21288588020652288No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT15420.1009440428285542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47600.084.777561
TAGGGTA5150.078.4861764
GTACGGG3350.077.16492
GTAGGGT5000.076.140723
TGTAGGG12400.075.807172
AGTAGGG26600.074.5646062
ACGGGTA1800.073.111794
TAGGGCA9750.072.790434
CGTAGGG3800.071.737522
TAGGGAC7550.071.590084
AGGGATG19100.071.361925
ATGTAGG5750.071.1626361
ATAGGGC10750.070.828573
TAATAGG4650.070.8020251
GTAGGGC7450.070.658393
GTAGGGA13650.070.242423
GGTAAGG9150.069.906631
ATAGCGG3300.069.836541
AAGGGCG5900.069.305734
GACCGAT950.069.259288
Produced by FastQC (version 0.11.5)