FastQCFastQC Report
Mon 27 Feb 2023
SRR3126569.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126569.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891946
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143350.7576854730526136No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27390.14477157381870306No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23580.12463357833680241No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG20430.10798405451318378Illumina PCR Primer Index 8 (95% over 22bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGGGAAGC20250.10703265315183413No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50550.083.8088761
ACGGGTA2700.074.8427354
ATAGCGG3900.074.833451
TAGGGCA12500.074.814894
AGGGATG41600.073.089665
ACGGGAT5750.072.738974
CGTAGGG6800.072.6088262
AGGGCAT17150.071.793015
TAGCGGG10550.071.314292
AGTAGGG32750.071.072722
GTACGGG5050.070.7670752
GAGGGAT31700.069.527814
GGATGGC32500.069.406937
AAGAGGG63200.069.269442
AAGGGAT36550.069.1735154
GTAGGGC10900.068.982443
AGGGACT28000.068.980895
GTAGGGA15400.068.0501563
TAGAGGG36200.067.936342
ATAGGGA20800.067.780213
Produced by FastQC (version 0.11.5)