FastQCFastQC Report
Mon 27 Feb 2023
SRR3126561.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126561.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282859
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203960.8934410754234055No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50130.21959306290927297No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40730.178416625818765No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC35920.1573465553501114No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG35910.15730275062980237No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG30730.13461190550971391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67350.084.366321
ACGGGTA2600.077.7289354
AGGGATG43100.077.313725
ATAGCGG4050.076.6529161
TAACGGG7400.074.9441762
TAGGGCA11150.074.608114
ATAGGGA20250.073.8056643
GTAGGGC11350.071.6370853
AGAGGGC33400.071.624063
TAAGGGA19500.071.34193
AAGGGTA14250.070.9106144
AGTAGGG38600.070.863622
AGGGAAT27950.070.792615
GGTAAGG14300.070.391051
GGATGGC36250.069.751317
ACGGGAT7150.069.676684
AGTAAGG14650.068.709351
TAGCGGG13200.068.362032
AAGGGAT43950.068.118994
GTACGGG6850.067.9254152
Produced by FastQC (version 0.11.5)