FastQCFastQC Report
Mon 27 Feb 2023
SRR3126555.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126555.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2831458
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255520.9024325983292No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT67550.23856966976024369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85900.084.318871
ATAGGGC16850.076.706263
CGTAGGG6900.073.565252
TAGGGCA14900.072.550364
ATTACGG3400.071.931921
AGTAGGG45350.071.821422
GTAGGGC12800.070.867223
TAGAGGG50950.070.8459552
ATAGGGA26100.069.869763
AGGGATG39650.069.462825
AGGGCAT24700.069.453475
ATAGAGG19100.069.1942751
ATAGCGG5850.067.5337141
TATAGGG29000.067.096582
GTAGGGA22200.066.9009253
CGAAGGG28200.066.6666952
AGGGACT48300.066.6563345
AGAGGGC47350.066.405523
AATAGGG42200.066.045052
ATAAGGG38450.066.007832
Produced by FastQC (version 0.11.5)