FastQCFastQC Report
Mon 27 Feb 2023
SRR3126553.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126553.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731234
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247320.9055247554768284No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49440.18101707872705158No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG27820.10185872027076406TruSeq Adapter, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81500.083.685111
AGTAGGG42900.074.052112
ATAGGGC18050.071.339383
GGTAAGG16000.070.261221
ATAAGGG33050.068.5367662
ACGGGAT5800.067.252144
ATGAGGG40450.066.9193952
GTAGGGT10350.066.747193
ATAGAGG17450.066.5793151
CGTAGGG7000.066.4638062
TGTAGGG19650.066.246862
ACGGGTA2350.065.993644
GTAGGGA20300.065.747363
TAGGGTA8650.065.739334
TAGGGTC6600.065.5088354
AGGGATG33300.065.342055
AGGGAAT26650.065.246995
AAGAGGG91500.065.176132
AGAGGGC42800.065.003083
TAGTAGG12400.064.865271
Produced by FastQC (version 0.11.5)