FastQCFastQC Report
Mon 27 Feb 2023
SRR3126544.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126544.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1465714
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119670.8164621474585083No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47460.32380123270979194No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG32780.22364526776710872No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG29660.20235871391008067No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC27570.1880994518712382TruSeq Adapter, Index 27 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25520.17411309436902425No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGAGTTAC24240.1653801491969102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT43750.083.4170461
CGTAGGG4000.081.054082
TAGGGCA9650.079.8577654
TCCGCTA8150.077.3247151
GAGGGAT25100.077.3173454
GGTAAGG7550.077.240651
ATAGCGG2450.076.7831
AGGGATG29700.075.309735
CTACGAC8400.074.9594045
CGCTACG8450.073.959773
GGATGGC21400.073.346227
ATAGGGA19000.073.204663
TACGACC8600.072.672256
AGGGATC17150.072.607645
AGTAGGG22200.072.386722
TAGCGGG7500.070.795062
ATAGGGC11600.070.4842153
TAAGGGA16400.070.4842153
TAGGGAT16950.070.41494
AGTAAGG8150.070.4001161
Produced by FastQC (version 0.11.5)