FastQCFastQC Report
Mon 27 Feb 2023
SRR3126538.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126538.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076337
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33190.3083606714253993No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAATCACG24310.22585862977859164No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG19280.1791260543863121No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG17070.15859345167916739No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC16420.15255445088294836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT34400.085.8529051
CGTAGGG2300.079.6870652
CGAAGGG6600.079.0369342
AAGGGCG4600.076.625744
AAGGGCA16500.074.34094
AGGGCGA6150.074.125545
ATAGCGG2100.073.900681
TAAGCGG2100.073.900681
ATTGCGG1150.073.608581
GAAGGGC14250.073.546373
AGCGGGT4350.073.466773
GGGCGAT5200.073.2070546
ACGGGTC2000.072.8455354
AGCGGGC4650.072.769743
CGGGTAT1950.072.303275
ATAGGGC7850.071.842733
ACGGGAT3150.071.614664
CGGGTAG1450.071.305975
AGTACGG800.070.541561
GCGGGTA3200.070.4956744
Produced by FastQC (version 0.11.5)