FastQCFastQC Report
Mon 27 Feb 2023
SRR3126534.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126534.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033942
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121581.175888009192005No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG42290.4090171402264343No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC41250.3989585489321451No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG40480.3915113226854118No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32570.31500799851442346No Hit
GAATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT18460.17853999547363392No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTAAGTCCT16970.1641291291000849No Hit
AGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT14660.1417874503598848No Hit
AGAGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT12780.12360461225097734No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT12650.12234728833919117No Hit
AAAGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT11670.11286900038880324No Hit
AAATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT11390.11016091811726382No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT11230.10861344253352703No Hit
GGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT10440.10097278183882656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT43250.086.995451
TAGGGCG1900.086.57494
CGTAGGG3150.083.551642
ATAGCGG2100.082.865761
ACGGGAT2750.080.323514
GTACGGG2400.080.287912
AGGGAAT17450.078.913215
GGTAAGG4550.078.558811
ATGTAGG2850.077.561411
GTAGGGC4750.077.175343
AGGGATG18450.076.673925
GTAGGGT3600.075.718683
TAGGGTA3450.074.924034
TACGGGA2450.074.812823
TACGACC6850.074.784826
AGGGTAA4950.074.0571445
AGGGCGA3750.073.943215
TAGGGCA5450.073.2993244
AGTAGGG15300.073.107682
TAGCGGG5600.073.014442
Produced by FastQC (version 0.11.5)