FastQCFastQC Report
Mon 27 Feb 2023
SRR3126456.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126456.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2420413
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278691.1514150684201414No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31590.13051491625602737No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99300.087.250921
ATAGCGG4250.078.577661
CGTAGGG7950.073.896272
GTAGGGC13050.071.307353
ATAGGGC13450.070.933833
TAGGGCA12950.070.40634
AGGGATG38200.070.250985
GAGGGAT36200.070.237384
ACGGGAT8450.070.0798954
TATAGGG20150.069.9722442
TAGAGGG52000.068.32792
AGGGACT33400.067.260655
AGTAGGG45400.067.18442
ACGGGTA2100.067.140044
ATGCGGG13700.066.8950042
GTAGGGA19250.066.651763
ATAGGGA22450.066.5718153
TAAGGGA21400.066.3243263
AGGGCAT19400.065.6518865
TAGACGG4300.065.631521
Produced by FastQC (version 0.11.5)