FastQCFastQC Report
Mon 27 Feb 2023
SRR3126436.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126436.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645150
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216601.3165972707655837No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23310.14168920767103305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69850.086.999741
GTAGGGC7700.073.842623
TAGGGCA9650.071.581784
CGTAGGG4150.071.331032
AGGGCAT14650.070.8870855
AGTAGGG29000.069.995772
ATAGGGC9150.069.330633
GGATAGG10150.068.583171
ACGGGAT4700.067.9866264
AAGGGGC50450.067.43574
TATAGGG14950.067.260282
CGAAGGG19650.065.759152
TAGAGGG32400.065.6960452
AAGGGCA25650.065.402344
ATAGCGG3600.065.32651
AAGAGGG60050.064.7893142
ATAGAGG12250.064.505251
GAATAGG9700.064.4913941
AGTAAGG12650.064.3246461
GTAAGGG28350.064.307962
Produced by FastQC (version 0.11.5)