FastQCFastQC Report
Mon 27 Feb 2023
SRR3126394.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126394.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708499
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281041.03762268326479No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28850.10651656138695269No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100150.086.86481
ATAGCGG6000.076.022751
TAGGGCA18750.073.927654
ATAGGGC19250.073.7174763
ACGGGTA3600.073.0923164
ACGGGAT7500.072.048144
AGTAGGG42750.069.246632
TAGGGTA8700.069.1316454
TAGCGGG17500.068.7378852
GTAGGGC15150.067.9243853
AGGGCAT25700.067.647985
GAATAGG17550.067.254241
TAGGGCG4500.066.827264
ATAGAGG18300.066.8105851
TATAGGG22700.066.6536942
CGAAGGG24400.066.246482
TAGAGGG47050.066.213742
ATAGGGA23200.065.824813
TAGGGAT16650.065.754864
AGGGATG35450.065.345675
Produced by FastQC (version 0.11.5)