FastQCFastQC Report
Mon 27 Feb 2023
SRR3126387.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126387.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2223663
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198110.8909173737207482No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29810.13405808344160064No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG25750.1157999211211411No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25680.11548512521906422No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC24970.1122921953551415TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG23300.10478206454845002No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67750.085.547561
AGGGATG46150.075.1572345
TAGGGCA14050.073.9268344
ATAGCGG5400.073.179891
AAGGGAT42900.070.662424
GAATAGG11800.070.167641
ACGGGAT7100.070.1671144
TAAGGGA22550.070.0291443
AGGGAAT26050.069.641115
AGTAGGG39350.069.3932952
ATAGAGG17550.068.890961
TAAACGG2400.068.606151
AGTAAGG15100.068.229691
TAGAGGG44250.067.975562
CGTAGGG6950.067.624072
AGAGGGC34700.067.315183
GGATGGC41550.067.301127
GTACGGG5550.066.8990862
CGAAGGG22500.066.215992
TAGCGGG13000.066.159752
Produced by FastQC (version 0.11.5)